Effect of anaerobic gymnastics exercise on vascular endothelial growth factor in obese boys

Vahid Saleh; Roghayye Afroundeh; Marefat siahkohiyan; Asadollah Asadi

doi:10.14238/pi60.6.2020.293-302

Vahid Saleh Ph.D. student of Sport Physiology, Department of Physical Education and Sport Sciences, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
Roghayye Afroundeh Assistant professor of Physical Education and Sport Sciences, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
Marefat siahkohiyan Professor of Physical Education and Sport Sciences, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
Asadollah Asadi Associate Professor of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.

DOI: https://doi.org/10.14238/pi60.6.2020.293-302

Keywords: pediatric obesity, endothelial growth factors, anaerobic gymnastics, weight loss

Abstract

Background Vascular endothelial growth factor (VEGF) is the most important growth factor involved in angiogenesis and appears to be mediated through exercise training, leading to increased blood lactate.

Objective To evaluate and compare the effects of anaerobic gymnastics exercise (AGE) on systemic VEGF in obese and normoweight boys.

Methods Sixty boys aged 8 to 12 years who enrolled in elementary level of gymnastics participated in this study and were randomly divided into four groups of 15 subjects each: obese AGE, obese control, normoweight AGE, and normoweight control. The control group didnâ€™t have any exercise during the study. The experimental groups performed 45 minutes of AGE 3 times per week for 8 weeks, which included a 10-minute warm-up, 30-minute main exercises, and 5-minute cool down. Body composition characteristics and VEGF levels in saliva were measured before and after 8 weeks of training.

Results Significant changes following AGE were found in the obese group in terms of weight (-8.09%; P=0.001), body fat% (BF%) (-12.81; P=0.001), body fat weight (BFW) (-19.38; P=0.001), and lean body weight (LBW) (-3.20; P=0.001). Saliva levels of VEGF increased post-AGE in the obese (+21.64%; P=0.79) and normoweight groups (+28.22; P=0.06), but the differences were not significant. Significant differences in weight, BF%, and BFW were found in between obese AGE group with obese control, normoweight control and normoweight AGE (PË‚0.05).

Conclusion Circulating VEGF concentrations slightly increase after 8 weeks of AGE in obese and normal-weight groups. Moreover, we demonstrate that weight significantly decreased in obese children after they engaged in AGE training.

References

1. Leckie RL, Oberlin LE, Voss MW, Prakash RS, Szabo-Reed A, Chaddock-Heyman L, et al. BDNF mediates improvements in executive function following a 1-year exercise intervention. Front Hum Neurosci. 2014;8:985. DOI: 10.3389/fnhum.2014.00985. eCollection 2014.
2. Gustafsson T, Rundqvist H, Norrbom J, Rullman E, Jansson E, Sundberg CJ. The influence of physical training on the angiopoietin and VEGF-A systems in human skeletal muscle. J Appl Physiol. 2007;103:1012-20. DOI: 10.1152/japplphysiol.01103.2006.
3. Adams RH, Alitalo K. Molecular regulation of angiogenesis and lymphangiogenesis. Nat Rev Mol Cell Biol. 2007;464-78. DOI:10.1111/j.1600-0463.2004.apm11207-0807.x.
4. Bhattacharya R, Kwon J, Li X, Wang E, Patra S, Bida JP, et al. Distinct role of PLC?3 in VEGF-mediated directional migration and vascular sprouting. J Cell Sci. 2009;122:1025-34. doi: 10.1242/jcs.041913.
5. Chang S-H, Kanasaki K, Gocheva V, Blum G, Harper J, Moses MA, et al. VEGF-A induces angiogenesis by perturbing the cathepsin-cysteine protease inhibitor balance in venules, causing basement membrane degradation and mother vessel formation. Cancer Res. 2009;69:4537-44. DOI: 10.1158/0008-5472.CAN-08-4539.
6. Hudlicka O, Brown MD. Adaptation of skeletal muscle microvasculature to increased or decreased blood flow: role of shear stress, nitric oxide and vascular endothelial growth factor. J Vasc Res. 2009;46:504-12. DOI: 10.1159/000226127.
7. Morland C, Andersson KA, Haugen ØP, Hadzic A, Kleppa L, Gille A, et al. Exercise induces cerebral VEGF and angiogenesis via the lactate receptor HCAR1. Nat Commun. 2017;8:15557. DOI: 10.1038/ncomms15557.
8. Ding Y-H, Li J, Zhou Y, Rafols JA, Clark JC, Ding Y. Cerebral angiogenesis and expression of angiogenic factors in aging rats after exercise. Curr Neurovasc Res. 2006;3:15-23. DOI: 10.2174/156720206775541787.
9. Brixius K, Schoenberger S, Ladage D, Knigge H, Falkowski G, Hellmich M, et al. Long-term endurance exercise decreases antiangiogenic endostatin signaling in overweight men aged 50–60 years. Br J Sports Med. 2008;42:126-9. DOI.org/10.1136/bjsm.2007.035188.
10. Disanzo BL, You T. Effects of exercise training on indicators of adipose tissue angiogenesis and hypoxia in obese rats. Metabolism. 2014;63:452-5. DOI: 10.1016/j.metabol.2013.12.004.
11. Duggan C, de Dieu Tapsoba J, Wang C-Y, McTiernan A. Dietary weight loss and exercise effects on serum biomarkers of angiogenesis in overweight postmenopausal women: a randomized controlled trial. Cancer Res. 2016;76:4226-35. DOI: 10.1158/0008-5472.CAN-16-0399.
12. Cullberg KB, Christiansen T, Paulsen S, Bruun J, Pedersen S, Richelsen B. Effect of weight loss and exercise on angiogenic factors in the circulation and adipose tissue in obese subjects. Obesity. 2013;21:454-60. DOI: 10.1002/oby.20060.
13. Paillard T, Rolland Y, de Souto Barreto P. Protective effects of physical exercise in Alzheimer's disease and Parkinson's disease: a narrative review. J Clin Neurol. 2015;11:212-9. DOI: 10.3988/jcn.2015.11.3.212.
14. Ferrara N. Vascular endothelial growth factor and the regulation of angiogenesis. Recent Prog Horm Res. 2000;15-35; discussion 35-6. PMID: 11036931.
15. Cao Y. Angiogenesis modulates adipogenesis and obesity. J Clin Invest. 2007;117:2362-8. DOI: 10.1172/JCI32239.
16. García de la Torre N, Rubio MA, Bordiu E, Cabrerizo L, Aparicio E, Hernández C, et al. Effects of weight loss after bariatric surgery for morbid obesity on vascular endothelial growth factor-A, adipocytokines, and insulin. J Clin Endocrinol Metab. 2008;93:4276-81. DOI: 10.1210/jc.2007-1370.
17. Silha J, Krsek M, Sucharda P, Murphy L. Angiogenic factors are elevated in overweight and obese individuals. Int J Obes. 2005;29:1308-14. DOI.org/10.1038/sj.ijo.0802987.
18. Duggan C, Xiao L, Wang C-Y, McTiernan A. Effect of a 12-month exercise intervention on serum biomarkers of angiogenesis in postmenopausal women: a randomized controlled trial. Cancer Epidemiol Biomarkers Prev. 2014;23:648-57. DOI: 10.1158/1055-9965.EPI-13-1155.
19. Jackson AS, Pollock ML. Generalized equations for predicting body density of men. Br J Nutr. 1978;40:497-504. DOI: 10.1079/bjn19780152.
20. Alves CRR, Borelli MTC, de Salles Paineli V, de Almeida Azevedo R, Borelli CCG, Junior AHL, et al. Development of a specific anaerobic field test for aerobic gymnastics. PloS One. 2015;10:e0123115. DOI: 10.1371/journal.pone.0123115.
21. ?ular D, Ivan?ev V, Zagatto AM, Mili? M, Beslija T, Sellami M, et al. Validity and reliability of the 30-s continuous jump for anaerobic power and capacity assessment in combat sport. Front Physiol. 2018;9:543. DOI: 10.3389/fphys.2018.00543.
22. Chiappin S, Antonelli G, Gatti R, De Palo EF. Saliva specimen: a new laboratory tool for diagnostic and basic investigation. Clin Chim Acta. 2007;383:30-40. DOI: 10.1016/j.cca.2007.04.011.
23. Richardson R, Wagner H, Mudaliar S, Saucedo E, Henry R, Wagner P. Exercise adaptation attenuates VEGF gene expression in human skeletal muscle. Am J Physiol Heart Circ Physiol. 2000;279:H772-H8. DOI: 10.1152/ajpheart.2000.279.2.H772.
24. Gavin TP, Wagner PD. Effect of short-term exercise training on angiogenic growth factor gene responses in rats. J Appl Physiol. 2001;90:1219-26. DOI: 10.1152/jappl.2001.90.4.1219.
25. Park J, Nakamura Y, Kwon Y, Park H, Kim E, Park S. The effect of combined exercise training on carotid artery structure and function, and vascular endothelial growth factor (VEGF) in obese older women. Japanese J Physical Fitness Sports Med. 2010;59:495-504. DOI: 10.7600/jspfsm.59.495.
26. Laughlin M, Roseguini B. Mechanisms for exercise training-induced increases in skeletal muscle blood flow capacity: differences with interval sprint training versus aerobic endurance training. J Physiol Pharmacol. 2008;59(Suppl 7):71-88. PMID: 19258658 PMCID: PMC2654584.
27. Goto C, Higashi Y, Kimura M, Noma K, Hara K, Nakagawa K, et al. Effect of different intensities of exercise on endothelium-dependent vasodilation in humans: role of endothelium-dependent nitric oxide and oxidative stress. Circulation. 2003;108:530-5. DOI: 10.1161/01.CIR.0000080893.55729.28.
28. Huang T, Larsen K, Ried?Larsen M, Møller N, Andersen LB. The effects of physical activity and exercise on brain?derived neurotrophic factor in healthy humans: a review. Scand J Med Sci Sports. 2014;24:1-10. DOI: 10.1111/sms.12069.
29. Meyer AA, Kundt G, Lenschow U, Schuff-Werner P, Kienast W. Improvement of early vascular changes and cardiovascular risk factors in obese children after a six-month exercise program. J Am Coll Cardiol. 2006;48:1865-70. DOI:org/10.1016/j.jacc.2006.07.035.
30. Shook RP, Hand GA, Wang X, Paluch AE, Moran R, Hébert JR, et al. Low fitness partially explains resting metabolic rate differences between African American and white women. Am J Med. 2014;127:436-42. DOI.org/10.1016/j.amjmed.2014.02.003.
31. Newton RL, Han H, Zderic T, Hamilton MT. The energy expenditure of sedentary behavior: a whole room calorimeter study. PLoS One. 2013;8:e63171. DOI: 10.1371/journal.pone.0063171.