Natural resistance-associated macrophage protein 1 gene polymorphisms in thalassemia patients with tuberculosis infection

  • Mohammad Ghozali Department of Biochemistry and Molecular Biology, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
  • Sari Puspa Dewi Department of Biochemistry and Molecular Biology, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
  • Reni Ghrahani Department of Child Health, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
  • Ani Melani Maskoen Health Research Unit, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
  • Lelani Reniarti Department of Child Health, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
  • Edhyana Sahiratmadja Department of Biochemistry and Molecular Biology, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
  • Tri Hanggono Achmad Department of Biochemistry and Molecular Biology, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.
DOI: https://doi.org/10.14238/pi56.2.2016.84-9
Keywords: NRAMP1, iron, tuberculosis, thalassemia

Abstract

that needs regular blood transfusions leading to accumulation of iron in the cells. This iron overload level in macrophage might cause intracellular bacteria, particularly Mycobacterium tuberculosis (MTB) to multiply. Polymorphisms in natural resistance-associated macrophage protein 1 (NRAMP1), a metal transporter across the phagosome membrane, play important role in regulating iron, which is also needed by MTB. Increased iron in thalassemia patients may have an increased potential risk for TB.
Objective To compare natural resistance-associated macrophage protein 1 (NRAMP1) gene polymorphisms (INT4, D543N, and 3’UTR) in thalassemia patients with and without tuberculosis (TB) infection.
Methods A cross-sectional measurement of NRAMP1 genetic polymorphisms was performed in pediatric thalassemia patients with TB (n=40) and without TB (n=50). Iron status including serum iron, total iron-binding capacity (TIBC), and ferritin, was compared between the two groups. The NRAMP1 genetic polymorphisms were analysed using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP). Allelic and genotypic distributions of each polymorphism were assessed for possible associations with TB infection.
Results Mean serum iron and TIBC in thalassemia patients with TB were higher compared to thalassemia patients without TB (mean serum: 166.26 vs. 134.92 μmol/L, respectively; P=0.026) and (mean TIBC: 236.78 vs. 195.84 μmol/L, respectively; P=0.029). In thalassemia patients with TB, we observed significantly higher frequency of the C allele in INT4 (10% vs. 2%, respectively; OR=5.44; 95%CI 1.1 to 26.4; P=0.02) and the TGTG deletion allele (78.8% vs. 51%, respectively; OR=3.56; 95%CI 1.83 to 6.9; P=0.0002) in 3’UTR polymorphisms than in thalassemia patients without TB. There were no significant 

differences in distributions of the A allele between TB and non-TB groups (16.3% vs. 15%, respectively; P=0.84) or the GA genotype (32.5% vs. 30%, respectively; P=0.79) in D543N.
Conclusion The NRAMP1 polymorphisms are known to be associated with major gene susceptibility to TB, and in our thalassemia patients this association is even more pronounced.

Author Biography

Mohammad Ghozali, Department of Biochemistry and Molecular Biology, Universitas Padjadjaran Medical School/Dr. Hasan Sadikin General Hospital, Bandung, West Java.

 

 

 

 

 

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Published
2016-07-19
How to Cite
1.
Ghozali M, Dewi SP, Ghrahani R, Maskoen AM, Reniarti L, Sahiratmadja E, Achmad TH. Natural resistance-associated macrophage protein 1 gene polymorphisms in thalassemia patients with tuberculosis infection. PI [Internet]. 19Jul.2016 [cited 5May2024];56(2):84-. Available from: https://www.paediatricaindonesiana.org/index.php/paediatrica-indonesiana/article/view/114
Issue
Vol 56 No 2 (2016): March 2016
Section
Pediatric Hemato-Oncology

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Received 2016-07-19
Accepted 2016-07-19
Published 2016-07-19